Workflow for Microplastics Analysis with Py-GC/MS System
Step 1 Sampling and pretreatment
Microplastics are extracted from environmental samples by appropriate
pretreatment and then homogenized using Cryogenic Mill (IQ-MILL 2070E) or
a mixer. The sample is then placed in a sample cup and weighed using a semimicro balance.
Step 2 Set of the sample cup on the Auto-Shot Sampler
The sample cups are placed onto the Auto-Shot Sampler. Up to 48 cups can be
loaded each time.
Step 3 Pyrolysis of the sample
The sample is introduced into the Micro-Furnace pyrolyzer by the Auto-Shot
Sampler and is pyrolyzed. The plastics become pyrolysis products (pyrolyzates)
and are introduced into the GC directly. On the other hand, the inorganics in the
sample remain in the sample cup as residues.
Step 4 GC/MS analysis
Pyrolyzates are separated on a GC column and detected by a single
Quadrupole mass spectrometer. The resulting pyrogram generally includes a
peak for each pyrolyzate. The mass spectrum from each peak reflects the
chemical structure of each pyrolyzate. Each polymer generates a unique set of
pyrolyzates.
Step 5 Data analysis
Reliable and quick Data analysis can be done using F-Search MPs 2.0.
Calibration curves are created automatically based on the analytical results from
the reference polymer mixture. Then F-Search MPs 2.0 performs quantitative
calculation automatically and results are reported for each sample.